The disappearance of the early colonizing species may be explained by antibiotic treatments or by competition between co-colonizing microorganisms. aeruginosa ( Folkesson et al., 2012) which progressively evolves toward a chronic colonization, that entails progressive lung tissue damage and impairment of pulmonary function. At the age of 20, 60–70% of CF-patients present intermittent colonization by P. predominate in older patients ( Goss and Burns, 2007 Cox et al., 2010 Lipuma, 2010). The composition of the microbial respiratory flora of cystic fibrosis (CF) patients changes with age Haemophilus influenzae and Staphylococcus aureus being present mainly in young children, while Pseudomonas aeruginosa, S.
Manipulating these pathways could be used to interfere with bacterial interactions and influence the colonization by respiratory pathogens. aeruginosa and three other respiratory pathogens involving several major metabolic pathways. This work identified both detrimental and beneficial interactions occurring between P. coli.Ĭonclusion: Complex interactions take place between the various bacterial species colonizing CF-lungs. This growth defect could be restored either by addition of biotin or by co-culturing the mutant in the presence of K. We identified a beneficial interaction in a bioA transposon mutant, unable to grow on rich medium. This mutant displayed a severe growth deficiency in the presence of Gram-negative but not of Gram-positive bacteria. We further identified a transposon insertion in purF, encoding a key enzyme of purine metabolism. pneumoniae but not Escherichia coli and S. The carB mutant was also unable to grow in the presence of B. PqsC is involved in the synthesis of 4-hydroxy-2-alkylquinolines (HAQs), a family of molecules having antibacterial properties, while carB is a key gene in pyrimidine biosynthesis. aureus growth, two transposon insertions located in pqsC and carB, resulted in reduced growth inhibition. Results: Whereas wild-type PA14 inhibited S. aeruginosa genes required for exploitative and direct competitions with S. Methods: We generated a library of PA14 transposon insertion mutants to identify P. aeruginosa and other co-colonizing pathogens including Staphylococcus aureus, Burkholderia sp., and Klebsiella pneumoniae may be crucial for pathogenesis and disease progression. Carmen H Logie, Natania Marcus, Ying Wang, Angela Kaida, Patricia O'Campo, Uzma Ahmed, Nadia O'Brien, Valerie Nicholson, Tracey Conway, Alexandra Pokomandy, Mylène Fernet, Mona Loutfy,, Aranka Anema, Denise Becker, Lori Brotto, Allison Carter, Claudette Cardinal, Guillaume Colley, Erin Ding, Janice Duddy, Nada Gataric, Robert S Hogg, Terry Howard, Shahab Jabbari, Evin Jones, Mary Kestler, Andrea Langlois, Viviane Lima, Elisa Lloyd‐Smith, Melissa Medjuck, Cari Miller, Deborah Money, Gina Ogilvie, Sophie Patterson, Neora Pick, Eric Roth, Kate Salters, Margarite Sanchez, Jacquie Sas, Paul Sereda, Marcie Summers, Christina Tom, Lu Wang, Kath Webster, Wendy Zhang, Rahma Abdul‐Noor, Jonathan Angel, Fatimatou Barry, Greta Bauer, Kerrigan Beaver, Anita Benoit, Breklyn Bertozzi, Sheila Borton, Tammy Bourque, Jason Brophy, Ann Burchell, Allison Carlson, Lynne Cioppa, Jeffrey Cohen, Curtis Cooper, Jasmine Cotnam, Janette Cousineau, Brenda Gagnier, Claudine Gasingirwa, Saara Greene, Trevor Hart, Shazia Islam, Logan Kennedy, Desiree Kerr, Maxime Kiboyogo, Gladys Kwaramba, Lynne Leonard, Johanna Lewis, Shari Margolese, Marvelous Muchenje, Mary, Ndung'u, Kelly O'Brien, Charlene Ouellette, Jeff Powis, Corinna Quan, Janet Raboud, Anita Rachlis, Edward Ralph, Sean Rourke, Sergio Rueda, Roger Sandre, Fiona Smaill, Stephanie Smith, Tsitsi Tigere, Wangari Tharao, Sharon Walmsley, Wendy Wobeser, Jessica Yee, Mark Yudin, Dada Mamvula Bakombo, Jean‐Guy Baril, Nora Butler Burke, Pierrette Clément, Janice Dayle, Danièle Dubuc, Danielle Groleau, Aurélie Hot, Marina Klein, Carrie Martin, Lyne Massie, Brigitte Ménard, Nadia O'Brien, Joanne Otis, Doris Peltier, Alie Pierre, Karène Proulx‐Boucher, Danielle Rouleau, Édénia Savoie, Cécile Tremblay, Benoit Trottier, Sylvie Trottier, Christos Tsoukas, Jacqueline Gahagan, Catherine Hankins, Renee Masching, Susanna Ogunnaike‐Cooke A longitudinal study of associations between HIV‐related stigma, recent violence and depression among women living with HIV in a Canadian cohort study, Journal of the International AIDS Society 22, no.77 (Jul 2019).Background: Chronic airway infection by Pseudomonas aeruginosa considerably contributes to lung tissue destruction and impairment of pulmonary function in cystic-fibrosis (CF) patients.
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